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Beschreibung
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Vorteile
PC-Erlenmeyerkolben – Die ideale Lösung für Ihre Laborbedürfnisse
The PC Erlenmeyer Flask is a premium autoclavable shake flask engineered from medical-grade polycarbonate (PC) for applications requiring exceptional mechanical strength, high-temperature resistance, and repeated sterilization cycles. Pre-sterilized by gamma irradiation and manufactured under cGMP conditions, these flasks are ideal for harsh bioprocessing environments, extreme pH conditions, and applications where glass-like durability meets plastic convenience.
Perfect for: High-density CHO cell culture, extreme pH protein expression, autoclave-required protocols, repeated sterilization cycles, high-temperature applications (up to 121°C), alkaline cell lysis, and demanding pharmaceutical manufacturing environments.
⚡ Why Choose Polycarbonate Over PETG?
- Autoclavable - Withstands 121°C repeated steam sterilization cycles
- Superior Impact Resistance - 250× stronger than glass, virtually shatter-proof
- Extreme Chemical Resistance - Handles pH 2-12, alkaline solutions, mild acids
- High Temperature Stable - Maintains integrity from -40°C to +135°C
- Reusable or Single-Use - Cost-effective for repeated use or disposable convenience
- Glass Alternative - Eliminates breakage hazards while maintaining optical clarity
✓ cGMP Quality Assurance
- Gamma Irradiated Sterile - 25-45 kGy sterilization (SAL 10⁻⁶)
- USP Class VI Certified - Medical-grade biocompatible polycarbonate
- cGMP Manufactured - ISO 13485 certified production facility
- DNase/RNase Free - No enzymatic contamination
- Pyrogen-Free - Tested to <0.25 EU/mL (LAL test)
- BSE/TSE Free - Animal-origin free raw materials
- Full Documentation - CoA, CoI, RSF, autoclave validation data available
🔬 Polycarbonate Advantages - Engineering for Excellence
Autoclavable - True Steam Sterilization Capability
Unlike PETG or standard plastics, polycarbonate maintains structural integrity at 121°C, enabling repeated autoclave sterilization cycles without deformation, warping, or loss of optical clarity. This makes PC flasks ideal for:
- Reusable applications - Autoclave and reuse up to 50+ times with proper care
- Cost reduction - Lower per-use cost compared to single-use PETG for high-volume operations
- Protocol compliance - Required for certain regulatory protocols mandating steam sterilization
- Media sterilization - Autoclave media directly in the flask, reducing transfer steps
- Contamination elimination - Terminal sterilization ensures absolute sterility
Autoclave Guidelines: Standard cycle: 121°C for 20 minutes at 15 PSI. Allow slow cooling to prevent thermal shock. Cap should be loosened during autoclave cycle.
Exceptional Impact Strength - 250× Stronger Than Glass
Polycarbonate is renowned as one of the toughest transparent thermoplastics available. PC flasks are virtually indestructible under normal lab conditions, providing:
- Safety improvement - Eliminates glass breakage injuries and contamination from shattered vessels
- Shipping resilience - Survives rough handling during transport without damage
- Drop resistance - Withstands accidental drops from bench height without cracking
- High-throughput compatible - Safe for automated handling systems and robotic platforms
- Education-friendly - Ideal for teaching labs where glass breakage is a concern
Superior Chemical Resistance - Handles Extreme Conditions
PC offers dramatically enhanced chemical resistance compared to PETG, particularly for alkaline and extreme pH applications:
✅ Compatible with:
- Alkaline solutions - NaOH up to 1M, KOH, ammonia solutions (pH 2-12)
- Mild acids - Dilute HCl, phosphoric acid, acetic acid
- Cell lysis buffers - Alkaline lysis solutions for protein extraction
- Cleaning agents - Detergents, Terg-a-Zyme, Alconox
- Alcohols - Methanol, ethanol (up to 70% for short-term exposure)
- Oxidizing agents - Dilute hydrogen peroxide, hypochlorite (bleach)
- Salts & buffers - All standard cell culture and bioprocess buffers
⚠️ Not recommended for:
- Strong acids - Concentrated HCl, H₂SO₄, HNO₃
- Organic solvents - Acetone, chloroform, DMF, DMSO (causes stress cracking)
- Aromatic hydrocarbons - Benzene, toluene, xylene
High Temperature Stability - From Freezing to Autoclave
PC flasks function across an extraordinary temperature range:
- -40°C - Safe for -80°C freezer storage without brittleness
- 4°C - Cold room storage for prepared media
- 37°C - Standard cell culture incubation
- 50-70°C - Heated mixing and dissolution applications
- 121°C - Autoclave sterilization (20 minutes)
- 135°C - Brief exposure for specialized sterilization protocols
Excellent Optical Clarity - Visual Culture Monitoring
While slightly less transparent than PETG (approximately 88% vs 92% light transmission), PC flasks maintain excellent optical clarity sufficient for:
- Visual assessment of cell density and culture turbidity
- pH indicator monitoring (phenol red color changes)
- Contamination detection (cloudiness, particulates)
- Media color verification
- Microscopic examination through flask wall (low magnification)
Note: For applications requiring maximum optical clarity (fluorescence microscopy, detailed imaging), PETG flasks may be preferred.
Vent Cap & Seal Cap Options - Application-Specific Flexibility
🌬️ Vent Cap (0.2μm Hydrophobic Membrane)
Best for: Standard cell culture applications
- ✓ Sterile gas exchange (O₂/CO₂)
- ✓ Prevents evaporation during extended culture
- ✓ Blocks bacteria, fungi, mycoplasma
- ✓ Suitable for 7-14 day cultures
- ✓ Maintains sterility during shaking
- ✓ Ideal for: CHO cells, HEK293, hybridomas
🔒 Seal Cap (Completely Closed)
Best for: Storage, transport, autoclaving
- ✓ Complete liquid-tight seal
- ✓ No gas exchange (anaerobic possible)
- ✓ Prevents contamination during transport
- ✓ Autoclave-compatible (loosen before cycle)
- ✓ Long-term media storage at 4°C
- ✓ Ideal for: Media prep, buffer storage, transport
📊 Technical Specifications
| Material | Medical-grade Polycarbonate (PC) - USP Class VI certified |
| Design | Conical (Erlenmeyer shape), Plain bottom |
| Sterilization Method | Gamma irradiation (25-45 kGy, SAL 10⁻⁶) - Autoclavable for reuse |
| Cap Options | Vent cap (0.2μm hydrophobic membrane) OR Seal cap (liquid-tight) |
| Available Sizes | 125mL, 250mL, 500mL, 1000mL (1L) |
| Working Volume | 125mL: 25-50mL | 250mL: 50-100mL | 500mL: 100-200mL | 1L: 200-400mL |
| Graduations | Molded-in volume markings (±5% accuracy) |
| Temperature Range | -40°C to +135°C (autoclave: 121°C for 20 min) |
| Autoclave Cycles | 50+ cycles tested without degradation |
| Impact Strength | 850 J/m (Izod) - 250× stronger than glass |
| Light Transmission | 88% (excellent clarity for visual monitoring) |
| Chemical Resistance | Excellent to pH 2-12, alkaline solutions, mild acids, alcohols |
| Shaker Compatibility | Orbital shakers (50-300 RPM), 25mm throw |
| Quality Certifications | DNase/RNase free, Pyrogen-free, Non-cytotoxic, BSE/TSE free |
| Packaging | Individually wrapped, double-bagged for cleanroom compatibility |
| Shelf Life | 5 years from manufacturing date (single-use) / Indefinite (reusable with proper care) |
| Storage Conditions | Room temperature (15-30°C), avoid UV exposure (may cause yellowing) |
📐 Dimensional Specifications
| Size | Total Capacity | Working Volume | Neck Diameter | Height | Base Diameter |
|---|---|---|---|---|---|
| 125mL | 125mL | 25-50mL | 38mm | ~95mm | ~65mm |
| 250mL | 250mL | 50-100mL | 38mm | ~120mm | ~80mm |
| 500mL | 500mL | 100-200mL | 38mm | ~165mm | ~105mm |
| 1000mL (1L) | 1000mL | 200-400mL | 38mm | ~220mm | ~130mm |
🎯 Applications - When to Choose PC Over PETG
🔥 High-Temperature Applications
- Autoclave sterilization required - Protocols mandating steam sterilization at 121°C
- Hot media preparation - Dissolving heat-labile compounds at 50-70°C
- Thermal lysis - Heat-based cell disruption protocols
- Repeated sterilization cycles - Cost-effective reuse for high-volume operations
🧪 Extreme pH & Chemical Conditions
- Alkaline protein extraction - Cell lysis with NaOH-based buffers (pH 10-12)
- Alkaline phosphatase reactions - High pH enzyme assays
- Cleaning validation studies - Detergent and bleach compatibility testing
- pH-extreme fermentations - Alkalophilic or acidophilic microorganisms
💪 High-Impact & Safety-Critical Environments
- Automated systems - Robotic handling where breakage risk is high
- Teaching laboratories - Student environments where glass breakage is common
- Field work - Mobile labs, field sampling, transport-intensive applications
- GMP manufacturing - Eliminating glass contamination risk in production areas
♻️ Reusable Cost-Effective Operations
- High-volume screening - 50+ autoclave cycles make per-use cost extremely low
- Process development - Repeated experiments with same vessels reduce variable costs
- Sustainability initiatives - Reduce single-use plastic waste
- Budget-conscious labs - Lower long-term costs compared to disposables
🧬 Specific Cell Culture Applications
- High-density CHO culture - Robust vessel for fed-batch processes (10+ days)
- Aggressive cell lines - Cells producing harsh metabolic byproducts
- Alkaline-adapted cells - Culture at pH 7.6-8.0
- Thermophilic organisms - Culture above 37°C (up to 70°C)
⚡ PC vs PETG - Detailed Comparison
Packaging:
Min package: 1 pcs/pack
Sales Package: 24 packs/case
Pcs/Case: (24 pcs total per case)
Carton size: 43 X 32 X 12
Gross weight: 1.5 kg
| Property | Polycarbonate (PC) | PETG |
|---|---|---|
| Autoclavable | ✅ Yes (121°C, 50+ cycles) | ❌ No (deforms at 70°C) |
| Impact Resistance | ✅ Excellent (850 J/m) | ⚠️ Good (100 J/m) |
| Alkaline Resistance (pH>10) | ✅ Excellent | ❌ Poor (degrades) |
| Optical Clarity | ⚠️ Good (88% transmission) | ✅ Excellent (92% transmission) |
| Temperature Range | ✅ -40°C to +135°C | ⚠️ -40°C to +70°C |
| Reusability | ✅ 50+ autoclave cycles | ❌ Single-use only |
| Cost per Unit | ⚠️ Higher initial | ✅ Lower initial |
| Cost per Use (50 cycles) | ✅ Much lower | ❌ Higher (50× units needed) |
| Organic Solvent Resistance | ❌ Poor (stress cracking) | ⚠️ Moderate |
Hohe Qualität und Sterilität
Die PC-Erlenmeyerkolben sind aus hochwertigem Polycarbonat gefertigt, was nicht nur ihre Haltbarkeit erhöht, sondern auch sorgt, dass sie steril sind. Da sie gamma-bestrahlt sind, erfüllen diese Kolben die höchsten Anforderungen an Hygiene. Daher sind sie optimal für Anwendung in Laboren, in denen steriles Arbeiten unerlässlich ist. Mit diesen Kolben können Sie sicher sein, dass Ihre Proben in einer einwandfreien Umgebung behandelt werden.
Autoklavierbar für vielseitigen Einsatz
Ein großer Vorteil der PC-Erlenmeyerkolben ist ihre Autoklavierbarkeit. Dies bedeutet, dass Sie die Kolben einfach sterilisierten können, ohne dass ihre Struktur oder Funktionalität darunter leidet. So können Sie die Kolben wiederholt verwenden, was sowohl kosteneffizient als auch umweltfreundlich ist. Diese Vielseitigkeit macht sie für verschiedene Anwendungen geeignet, wie z.B. im Bereich der Biotechnologie und Pharmaindustrie, wo häufig wechselnde Anforderungen bestehen.
cGMP-konform für höchste Ansprüche
Unsere PC-Erlenmeyerkolben sind cGMP-konform, was bedeutet, dass sie den aktuellen Good Manufacturing Practices entsprechen und somit für den Einsatz in regulierten Bereichen bestens geeignet sind. Das stellt sicher, dass alle produzierten Materialien von gleichbleibend hoher Qualität sind. So können Sie diese Kolben bedenkenlos in Ihrem Labor einsetzen, egal ob in der Forschung oder Produktion, und sicher sein, dass Sie stets die besten Ergebnisse erzielen.