PETG-Erlenmeyerkolben: Ideal für Ihre Laboranforderungen

The PETG Erlenmeyer Flask is a sterile, single-use shake flask engineered for suspension cell culture, media preparation, and bioprocess scale-up. Manufactured from USP Class VI medical-grade PETG (Polyethylene Terephthalate Glycol-modified) and sterilized by gamma irradiation, these conical flasks provide exceptional optical clarity, mechanical strength, and chemical resistance for demanding biomanufacturing applications.

Perfect for: CHO cell expansion, hybridoma culture, insect cell protein production, monoclonal antibody manufacturing, vaccine production, media preparation, and shaker culture applications in research and cGMP environments.

🔬 Why PETG is Superior for Cell Culture Applications

Crystal-Clear Optical Clarity - Monitor Cultures Visually

PETG material provides exceptional transparency comparable to glass, allowing easy visual inspection of cell density, morphology, and media color without opening the flask. This optical clarity is critical for monitoring culture health, detecting contamination early, and assessing readiness for harvest—all without compromising sterility.

Superior Mechanical Strength - Shatter-Resistant & Lightweight

Unlike fragile glass Erlenmeyer flasks that pose safety hazards, PETG flasks are virtually shatter-proof while weighing 90% less than equivalent glass vessels. This dramatically reduces shipping costs, handling injuries, and contamination risks from broken glass. Ideal for high-throughput operations where dozens of flasks are handled daily.

Excellent Chemical Resistance - Compatible with Standard Media & Buffers

PETG demonstrates outstanding resistance to:

• Cell culture media - DMEM, RPMI, Ham's F-12, serum-containing and serum-free formulations
• Buffers - PBS, HEPES, Tris, phosphate buffers (pH 6-8)
• Salts - Sodium chloride, potassium chloride, calcium chloride
• Supplements - Glutamine, amino acids, vitamins, growth factors
• Antibiotics - Penicillin, streptomycin, amphotericin B

Note: PETG is not compatible with strong acids, bases (pH >10), ketones, or aromatic hydrocarbons. For applications requiring extreme chemical resistance, consider our polycarbonate (PC) flask options.

Conical Bottom Design - Optimized for Suspension Culture

The classic conical Erlenmeyer shape provides the ideal geometry for orbital shaker applications:

• Optimal mixing pattern - Creates efficient circular fluid motion at 80-150 RPM
• Enhanced oxygen transfer - Large surface-to-volume ratio maximizes gas exchange
• Reduced shear stress - Smooth conical walls minimize damage to sensitive cells
• Easy harvesting - Narrow neck facilitates complete cell suspension recovery

Seal Cap with 0.2μm Hydrophobic Membrane - Sterile Gas Exchange

Each flask features a high-quality HDPE seal cap with integrated 0.2μm hydrophobic membrane filter:

• Sterile gas exchange - Allows CO₂/O₂ equilibration while blocking microorganisms (bacteria, fungi, mycoplasma)
• Prevents evaporation - Hydrophobic membrane blocks water vapor loss during extended culture (7-14 days)
• Secure liquid-tight seal - Prevents leakage during shaking at up to 250 RPM
• Easy handling - Wide-mouth opening compatible with standard pipettes and aseptic transfer equipment

📊 Technical Specifications

📐 Dimensional Specifications

🎯 Applications in Biomanufacturing & Research

🧬 Suspension Cell Culture - Scalable Protein Production

PETG Erlenmeyer flasks are the industry standard for suspension cell culture scale-up from bench to pilot scale:

• CHO cells (Chinese Hamster Ovary) - Recombinant protein and monoclonal antibody production for therapeutics
• HEK293 suspension cells - Transient protein expression, viral vector production (AAV, lentivirus)
• Hybridoma cells - Monoclonal antibody generation and small-scale mAb production
• Insect cells (Sf9, Sf21, High Five™) - Baculovirus expression system for recombinant proteins
• Yeast (Pichia, S. cerevisiae) - Industrial enzyme and biofuel production

💉 Vaccine & Viral Vector Manufacturing

Essential for small to medium-scale production of biologics:

• Influenza vaccine production - MDCK cells in suspension for seasonal flu vaccines
• Adenovirus production - Gene therapy vectors and oncolytic virus manufacturing
• Lentivirus production - CAR-T cell therapy and ex vivo gene editing applications
• Bacteriophage production - Phage therapy development

🧪 Media Preparation, Mixing & Storage

Versatile laboratory workhorse for routine applications:

• Culture media preparation - Dissolving powdered media, adding supplements, pH adjustment
• Buffer preparation - PBS, HEPES, Tris-buffered saline for downstream processing
• Solution mixing - Sterile blending of liquid components
• Short-term storage - Prepared media storage at 4°C (up to 4 weeks with seal cap)

🔬 Process Development & Optimization

Critical tool for bioprocess optimization:

• Cell line screening - Testing multiple clones in parallel for productivity
• Media optimization - Evaluating different media formulations and feed strategies
• Parameter screening - Testing pH, temperature, agitation rates, dissolved oxygen levels
• Scale-down models - Mimicking bioreactor conditions at bench scale (125mL-1L)
• Inoculum preparation - Seed train development for bioreactor inoculation

📚 Academic & Research Laboratories

Cost-effective solution for research applications:

• Protein expression studies - Characterizing new expression systems
• Cell biology research - Studying cell growth, metabolism, and signaling in controlled conditions
• Teaching laboratories - Demonstrating fermentation and cell culture principles
• Microbiology - Bacterial and fungal culture for research purposes

⚡ PETG vs Other Materials - Why PETG is Optimal

Recommendation: Choose PETG for standard suspension cell culture applications (pH 6-8, 37°C). For extreme pH conditions (>pH 10) or autoclave requirements, consider polycarbonate alternatives.

🔧 Usage Guidelines & Best Practices

Pre-Culture Preparation

1. Visual Inspection: Check packaging integrity and flask for cracks or defects before use
2. Equilibration: Allow flask to reach room temperature (15-20 minutes) to prevent condensation
3. Aseptic Handling: Open in biosafety cabinet or laminar flow hood using aseptic technique
4. Media Pre-warming: Warm culture media to 37°C before inoculation

Cell Inoculation & Culture

1. Working Volume: Use 20-40% of flask capacity for optimal oxygen transfer
   • 125mL flask → 25-50mL working volume
   • 250mL flask → 50-100mL working volume
   • 500mL flask → 100-200mL working volume
   • 1000mL flask → 200-400mL working volume
2. Seeding Density: Typically 2-5 × 10⁵ cells/mL for CHO, HEK293 suspension cells
3. Shaker Parameters:
   • Agitation rate: 100-150 RPM for most mammalian cells (adjust based on cell line)
   • Orbital throw: 25mm diameter (standard for most shakers)
   • Temperature: 37°C in humidified incubator
   • CO₂: 5-8% depending on media buffering system
4. Culture Duration: Typically 3-7 days for batch culture, up to 14 days for fed-batch with media supplementation

Monitoring & Maintenance

• Daily Visual Checks: Assess cell density, media color (pH indicator), and contamination signs
• Cell Counting: Sample 1-2mL aseptically every 1-2 days for cell density and viability measurement
• Glucose Monitoring: Track glucose consumption to optimize feeding strategy
• Tightness Check: Ensure cap remains securely sealed throughout culture to maintain sterility

Cell Harvest

1. Timing: Harvest at peak viability (typically 85-95%) before decline phase
2. Cell Recovery: Pour or pipette cell suspension directly from flask into collection vessel
3. Centrifugation: Spin at 300-500 × g for 5-10 minutes to pellet cells
4. Supernatant Collection: For protein/antibody harvest, collect clarified supernatant after centrifugation

Safety & Disposal

• Biosafety: Handle cell cultures according to institutional biosafety level (BSL-1 or BSL-2)
• Decontamination: Autoclave or treat with 10% bleach before disposal
• Recycling: PETG is recyclable (#1 plastic) where facilities accept medical plastics

📋 Quality Documentation & Regulatory Support

Complete cGMP Documentation Package

Every batch is accompanied by comprehensive quality documentation:

• Certificate of Analysis (CoA) - Batch-specific test results for all quality parameters
• Certificate of Irradiation (CoI) - Gamma dose report with min/max/average kGy values
• Material Safety Data Sheet (MSDS) - Safety information and emergency procedures
• Regulatory Support Files (RSF) - Manufacturing process documentation for regulatory submissions
• Biocompatibility Testing - USP Class VI cytotoxicity, sensitization, and irritation data
• Extractables & Leachables - Available upon request for IND/BLA filings
• Sterility Testing Reports - USP <71> validation per batch
• Endotoxin Testing - LAL test results (<0.25 EU/mL)
• BSE/TSE Certificates - Animal-origin free documentation

Regulatory Compliance Standards

• ✓ FDA 21 CFR Part

  Packaging:
  Min package: 1 pcs/pack
  Sales Package: 24 packs/case
  Pcs/Case: (24 pcs total per case)
  Carton size: 31 X 21 X 22
  Gross weight: 1.5 kg
  

Hochwertige Materialien für exakte Ergebnisse

Unsere PETG-Erlenmeyerkolben bestehen aus hochwertigem PETG-Material, das nicht nur transparent, sondern auch extrem stabil ist. Dieses Material ermöglicht eine ausgezeichnete Sichtbarkeit des Inhalts, weshalb Labormitarbeiter sofort die im Kolben befindlichen Flüssigkeiten erkennen können. Darüber hinaus sorgt die hohe chemische Beständigkeit des PETG dafür, dass der Kolben auch mit einer Vielzahl von Chemikalien verwendet werden kann, was ihn zu einem unverzichtbaren Werkzeug in jedem modernen Labor macht.

Sterilität und Sicherheitsstandards

Die PETG-Erlenmeyerkolben sind gamma-bestrahlt, was ihre Sterilität gewährleistet. Dies ist besonders wichtig für Anwendungen, bei denen Verunreinigungen vermieden werden müssen. Die Einhaltung von cGMP (Current Good Manufacturing Practice) Standards stellt sicher, dass diese Kolben höchsten Sicherheits- und Qualitätsanforderungen entsprechen. Laboranwender können sich darauf verlassen, dass sie mit unseren Erlenmeyerkolben sichere und hygienische Ergebnisse erzielen, die für zahlreiche Anwendungen in der Biotechnologie, Chemie und Pharmakologie geeignet sind.

Vielseitigkeit in der Anwendung

PETG-Erlenmeyerkolben sind nicht nur für die Kultivierung von Mikroben geeignet, sondern können auch für verschiedene chemische Reaktionen und umfassende Laborversuche eingesetzt werden. Diese Flexibilität macht sie sowohl für akademische als auch für industrielle Labore attraktiv. Darüber hinaus können sie problemlos in Schüttler eingesetzt werden, wodurch ihre Nutzung in der Forschung und Entwicklung noch effizienter wird. Weil sie leicht und langlebig sind, bieten sie zugleich hervorragende Benutzerfreundlichkeit und Anpassungsfähigkeit an verschiedene Laborbedingungen.