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Sterile Serological Pipettes Disposable | 1mL 2mL 5mL 10mL 25mL 50mL | Gamma Irradiated cGMP

Sterile Serological Pipettes Disposable | 1mL 2mL 5mL 10mL 25mL 50mL | Gamma Irradiated cGMP

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Sterile Serological Pipettes Disposable | 1mL...
Paper Bag /
10ml
Normaler Preis €55,90
Normaler Preis €55,90 Verkaufspreis €55,90
Stückpreis €0,11  pro  item
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Premium Sterile Disposable Serological Pipettes - Precision Liquid Handling for Cell Culture & Laboratory Applications

Our Sterile Disposable Serological Pipettes are manufactured from medical-grade polystyrene with precise bidirectional graduations for accurate liquid handling in cell culture, microbiology, and general laboratory applications. Gamma irradiated to ensure sterility and produced under cGMP conditions, these pipettes deliver reliable, consistent performance for pharmaceutical, biotech, and academic research laboratories.

Perfect for: Cell culture media transfer, reagent dispensing, sample preparation, microbiology applications, aseptic liquid handling, pharmaceutical manufacturing, quality control testing, and general laboratory use.

✓ cGMP Quality Assurance

  • Gamma Irradiated Sterile - 25-45 kGy sterilization (SAL 10⁻⁶)
  • USP Class VI Certified - Medical-grade biocompatible polystyrene
  • cGMP Manufactured - ISO 13485 certified production facility
  • DNase/RNase Free - No enzymatic contamination
  • Pyrogen-Free - <0.25 EU/mL (LAL test)
  • BSE/TSE Free - Animal-origin free raw materials
  • Non-Cytotoxic - Safe for sensitive cell culture applications
  • Full Documentation - CoA, CoI, RSF available for regulatory compliance

📏 Complete Size Range - 1mL to 50mL

Size Total Length Graduation Interval Accuracy (±) Color Code Packaging
1mL ~230 mm 0.01 mL ±0.01 mL Blue 200/bag, 1000/case
2mL ~230 mm 0.01 mL ±0.02 mL Green 200/bag, 1000/case
5mL ~295 mm 0.05 mL ±0.05 mL Yellow 200/bag, 1000/case
10mL ~325 mm 0.1 mL ±0.1 mL Orange 200/bag, 500/case
25mL ~360 mm 0.2 mL ±0.2 mL Red 100/bag, 400/case
50mL ~390 mm 0.5 mL ±0.5 mL Purple 100/bag, 200/case

💡 Color-Coded for Easy Identification: Each pipette size features a unique color band for quick visual identification, reducing errors in fast-paced laboratory environments.


🔬 Premium Design Features

✅ Bidirectional Graduations - Read from Top or Bottom

Our serological pipettes feature ascending and descending graduations printed in bold, easy-to-read numbers. This dual-scale design allows you to:

  • Measure dispensed volume - Read descending scale (TD - To Deliver)
  • Measure remaining volume - Read ascending scale from tip
  • Reduce calculation errors - Choose most convenient reading direction
  • Improve efficiency - No mental math required for partial volumes

✅ Negative Graduation Zone - Accurate "To Deliver" Marking

Each pipette includes a negative graduation zone below the zero mark, ensuring complete liquid delivery when emptying to the last drop. This is critical for:

  • Accurate cell culture media transfer
  • Precise reagent dispensing in assays
  • Standardization across experiments
  • Meeting pharmaceutical manufacturing tolerances

✅ Large-Bore Opening - Fast Flow Rate

The wide tip opening facilitates rapid liquid uptake and dispensing, making these pipettes ideal for:

  • High-throughput applications
  • Viscous solutions (serum, glycerol stocks)
  • Media containing particulates
  • Cell suspensions without clogging

✅ Cotton Plug Filter - Prevents Cross-Contamination

Each pipette features a built-in hydrophobic cotton plug in the mouthpiece that:

  • Prevents liquid from entering pipette controller or pipettor
  • Blocks aerosol contamination
  • Protects expensive pipetting devices from damage
  • Maintains sterility throughout use
  • Meets biosafety requirements for hazardous materials

✅ Color-Coded Bands - Quick Size Identification

Universal color coding system matches industry standards:

  • 🔵 Blue - 1mL
  • 🟢 Green - 2mL
  • 🟡 Yellow - 5mL
  • 🟠 Orange - 10mL
  • 🔴 Red - 25mL
  • 🟣 Purple - 50mL

✅ Crystal-Clear Polystyrene - Excellent Visibility

Medical-grade transparent polystyrene construction provides:

  • Easy visual inspection of liquid level
  • Detection of air bubbles
  • Verification of complete liquid transfer
  • Enhanced accuracy through clear graduation visibility

✅ Universal Compatibility - Fits All Pipette Controllers

Standard dimensions ensure compatibility with:

  • Manual pipette controllers (pipette pumps)
  • Electronic pipettors (motorized controllers)
  • Automated liquid handling systems
  • Multi-channel pipetting devices

📊 Technical Specifications

Material Virgin polystyrene (PS) - USP Class VI certified
Graduation Type Bidirectional (ascending & descending), negative graduation zone
Printing Method Screen-printed, permanent markings (will not fade or wash off)
Filter Type Hydrophobic cotton plug (prevents overflow and contamination)
Sterilization Method Gamma irradiation (25-45 kGy, SAL 10⁻⁶)
Accuracy Class Meets or exceeds ASTM E1293-20 standards
Tip Design Large-bore opening for fast flow and reduced clogging
Color Coding Universal ISO color bands for size identification
Temperature Range -10°C to +40°C (not autoclavable)
Quality Certifications DNase/RNase free, Pyrogen-free (<0.25 EU/mL), Non-cytotoxic, BSE/TSE free
Packaging Individually paper-plastic wrapped, bulk bagged, master carton
Shelf Life 5 years from manufacturing date when stored properly
Storage Conditions Room temperature (15-30°C), dry environment, away from direct sunlight
Single-Use Yes - disposable, not intended for reuse or re-sterilization

🎯 Applications Across Laboratory Disciplines

🧬 Cell Culture & Tissue Culture

  • Media transfer - Adding culture media to flasks, plates, and dishes
  • Cell feeding - Media changes for adherent cell cultures
  • Cell passaging - Transferring cell suspensions during subculture
  • Reagent addition - Dispensing growth factors, supplements, antibiotics
  • Serum addition - Accurately adding FBS or other sera to media
  • Trypsin/EDTA handling - Dispensing cell dissociation reagents
  • Suspension culture - Transferring suspension cells without damage

🔬 Microbiology & Clinical Diagnostics

  • Sample preparation - Serial dilutions, sample pooling
  • Media preparation - Dispensing liquid broth and agar
  • Inoculation - Transferring bacterial/fungal cultures
  • Clinical specimen handling - Blood, urine, CSF sample processing
  • Antimicrobial susceptibility testing - Accurate inoculum preparation
  • Mycology applications - Fungal culture work

💊 Pharmaceutical & Biotech Manufacturing

  • cGMP production - Aseptic liquid handling in cleanrooms
  • Quality control testing - Sterility testing, endotoxin assays
  • Raw material testing - Sampling and testing incoming materials
  • Buffer preparation - Accurate buffer component mixing
  • Formulation development - Drug product development work
  • Fill-finish operations - Sampling during vial filling

🧪 Molecular Biology & Biochemistry

  • Reagent dispensing - Enzymes, nucleotides, buffers
  • DNA/RNA work - Transferring nucleic acid solutions
  • Protein purification - Column loading and fraction collection
  • PCR setup - Master mix preparation
  • Western blotting - Buffer and antibody handling
  • ELISA assays - Reagent addition to microplates

🏫 Academic Research & Teaching Labs

  • Student experiments - Cost-effective for high-volume teaching
  • General liquid handling - Versatile for multiple applications
  • Chemical preparations - Solution making and transfers
  • Biological assays - Various biochemistry and cell biology experiments

🩺 Clinical & Hospital Laboratories

  • Blood banking - Serum separation and handling
  • Immunology - Antibody testing, cell typing
  • Hematology - Blood cell counts and morphology
  • Chemistry analyzers - Sample preparation

💡 How to Choose the Right Pipette Size

Pipette Size Best For Typical Applications
1mL Small volumes, precise work Microcentrifuge tube work, small-scale transfections, adding concentrated reagents
2mL 96-well plates, small flasks Filling 96-well plates, small T-flask work, reagent addition to tubes
5mL T25 flasks, 24-well plates Most common size for routine cell culture, media changes in T25 flasks
10mL T75 flasks, 6-well plates Standard cell culture work, media changes in T75 flasks, sample pooling
25mL T175 flasks, large volumes Large flask media changes, buffer preparation, bulk reagent dispensing
50mL T225 flasks, bottles Large-scale cell culture, media bottle filling, high-volume transfers

💡 Pro Tip: For best accuracy, choose a pipette size where your target volume falls in the middle 50-80% of the pipette's range. For example, use a 10mL pipette for 5-8mL volumes rather than a 25mL pipette.


🔧 Usage Guidelines & Best Practices

Proper Pipetting Technique

  1. Attach to Controller:
    • Remove pipette from wrapper using aseptic technique
    • Insert firmly into pipette controller or manual pump
    • Ensure secure fit to prevent leakage or detachment
  2. Aspirate Liquid:
    • Submerge tip 2-5mm below liquid surface
    • Aspirate slowly to avoid bubble formation
    • Keep pipette vertical (perpendicular to bench)
    • Do not allow liquid to reach the cotton plug filter
  3. Dispense Liquid:
    • Touch tip to vessel wall at 30-45° angle
    • Dispense slowly and smoothly
    • Allow final drops to drain naturally (3-5 seconds)
    • For "blow-out" pipettes, expel final drop using controller
  4. Disposal:
    • Discard in appropriate biohazard or sharps container
    • Never reuse single-use pipettes
    • Follow institutional biosafety guidelines

Common Mistakes to Avoid

  • Aspirating too fast - Causes bubbles and inaccurate volumes
  • Touching pipette tip to non-sterile surfaces - Compromises sterility
  • Overfilling past the cotton plug - Contaminates pipette controller
  • Holding pipette horizontally while filled - Liquid may reach filter
  • Not allowing drainage time - Results in inaccurate delivered volume
  • Using wrong size for volume - Reduces accuracy

Maintenance of Pipette Controllers

To protect your pipetting equipment when using serological pipettes:

  • Regularly check and replace controller filters
  • Never invert controller with pipette attached
  • Clean exterior with 70% ethanol regularly
  • If liquid enters controller, decontaminate immediately per manufacturer instructions
  • UV decontaminate controller between uses with hazardous materials

📋 Quality Documentation & Regulatory Support

Complete cGMP Documentation Package

Every batch includes comprehensive regulatory documentation:

  • Certificate of Analysis (CoA) - Batch-specific quality test results
  • Certificate of Irradiation (CoI) - Gamma sterilization validation with dose mapping
  • Material Safety Data Sheet (MSDS/SDS) - Safety and handling information
  • Regulatory Support Files (RSF) - Manufacturing process documentation
  • Biocompatibility Testing - USP Class VI cytotoxicity data
  • Sterility Testing Reports - USP <71> validation per batch
  • Endotoxin Testing - LAL test results (<0.25 EU/mL)
  • BSE/TSE Certificates - Animal-origin free raw material documentation
  • Accuracy Validation - ASTM E1293 compliance data

Regulatory Compliance Standards Met

  • ✓ ASTM E1293-20 (Standard Specification for Graduated Disposable Pipets)
  • ✓ ISO 8655 (Piston-operated volumetric apparatus)
  • ✓ FDA 21 CFR Part 210/211 (cGMP for Pharmaceuticals)
  • ✓ ISO 13485 (Medical Devices Quality Management)
  • ✓ USP <87> (Biological Reactivity Tests, In Vitro)
  • ✓ USP <71> (Sterility Tests)
  • ✓ ISO 10993 (Biological Evaluation of Medical Devices)
  • ✓ EU GMP Annex 1 (Sterile Medicinal Products)

Min package: 50pcs/pack
Sales Package: 10pcs/case
Pcs/Case: 500
Carton size: 41 X 31 X 27
Gross weight: 6.5 KG

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